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12.275  Articles
1 of 1.229 pages  |  10  records  |  more records»
DNA (deoxyribonucleic acid) extraction is the first key step in the success of a genetic analysis. This research aims to validate the optimal method of DNA extraction from a number of 30 blood samples collected from Holstein cattle breed. Two methods of D... see more

Objectives: Extraction of DNA and RNA is the first step in genomics and transcriptomics studies. Phenol-chloroform method for DNA extraction has been the widely used method. However, this method is relatively expensive and time-consuming. The objective of... see more

DNA extraction is usually the first step to perform molecular studies. This process can be nonviable due to genomic DNA (gDNA) extraction commercial kits prices. Furthermore, available DNA extraction protocols generally have high specificity, limiting the... see more

The authors report their difficulties in DNA extraction from ancient bones found in shell-mounds of Sta. Catarina Island, SC, Brazil. The presence of humic acid, fulvic acid, free oxidant radicals and the hydrolytic effect of wet environment degrade the D... see more

Background:  Sequence-based identification of various microorganisms including Archaea, Bacteria, Cyanobacteria, Diatoms, Fungi, and green algae necessitates an efficient and reproducible genome extraction procedure though which a pure template DNA i... see more

In this study, two methods for DNA extraction from fresh rat blood were compared. One is based on the use of cetyltrimethylammonium bromide (CTAB method), while the other one is well-known salting out method. Spectrophotometric analysis was employed to as... see more

This paper describes the process of DNA extraction and quantification of diploid and tetraploid red clover (Trifolium pratense L.). Red clover is an important forage crop for research purposes which has seen a gradual decline in use for a century. The pap... see more

. We tested three methods for DNA isolation and purification based on different principles: a CTAB extraction protocol; the Maxwell™ 16 Tissue DNA Purification Kit (Promega) which facilitates an automated extraction using magnetic beads; and the High Pure... see more

Leptin is a 16 kDa protein hormone mainly but not exclusively produced and secreted by adipose tissue, which functions as the afferent signal in a feedback loop regulating adipose tissue mass. The progress in animal breeding may be improved by combining t... see more

1 of 1.229 pages  |  10  records  |  more records»