Mapping of Multiple P{lacW} Insertions into the Germline of Drosophila melanogaster

SUMMARY

Artificial mobile elements are widely used as molecular tools for transposon mutagenesis in Drosophila melanogaster and precise mapping by inverse PCR is fundamental for describing of new insertional alleles. Transgenic lines harboring only one transposon copy/genome are usually constructed, so their genetic and molecular analysis is strightforward. Nevertheless, some experimental tasks may require generation of mutant lines hosting more than one transposon insertion/genome, a condition that may impair the accuracy of inverse PCR results. Here we describe a robust inverse PCR procedure that allows mapping of multiple P{lacW} insertions into the germline of D. melanogaster transgenic lines.