SUMMARY
Background: Science the discovery of antibiotic, the incidence of antibiotic resistance has been inevitable. Although there has been many study in these area but problem still exists. The aim of this research was to study the serotyping and multiple antibiotic resistance patterns of Shigella sonnei isolated from diarrheal stool of children. Methods: The stool samples of children from zero to fourteen years of age admitted at Children Medical Center in Tehran were tested over period of twelvemonth. Identification of isolates was carried out according to standard methods and the antibiotic susceptibility test was performed using Kirby Bauer disk method. Results: Of the 200 samples analyzed 6 (3%) were tested positive for Shigella sonnei. The antibiotic resistance patterns showed that all were résistance to tetracycline, streptomycin, clindamycin and cortimoxazol, and 66.7% of the samples had multiples resistance to above antibiotics. Conclusion: The results showed that multiple antibiotics resistance of Shigella sonnei is increasing, therefore awareness about the prevention by improved hygiene and proper medication are needed to reduce the burden of the preventable infectious diseases among young children.