SUMMARY
Statistically comparable method has been developed, validated, for estimating probenecid in tablet dosage form with the application of hydrotropic solubilization phenomenon. This research effort utilizes 2M sodium acetate as hydrotropic solubilizing agent which enhances the aqueous solubility of probenecid by 85 folds. The drug stability in hydrotropic solution was found to be more than 48hrs.The probenecid hydrotrope obeys the Beers Lambert law at maximum wavelength of 244.5nm. The drug response was found to be linear in the range of 2 26 g/ml with correlation coefficient of 0.9987.The non-interference of hydrotropic agent and additives in the course of probenecid estimation ensures the specificity of the proposed method.The mean recovery of 99.43% reflects its accuracy, obtained in good terms. The method precision results were found to be within limit expressed in %RSD (NMT 2%). The results of comparison of proposed method with standard method (IP 2010) were found to be in the similar range. The comparison provides evidence of proposed method to be simple, less time consuming, safe and eco-friendly. The validation (as per ICH 2005) of the proposed method accesses its use in the routinely quality control of probenecid in tablet dosage form.