ARTICLE
TITLE

Highly efficient enzymatic preparation of isomalto-oligosaccharides from starch with enzyme cocktail

SUMMARY

Background: Current commercial production of isomalto-oligosaccharides (IMOs) commonly involves a lengthy multistage process with low yields. Results: To improve the process efficiency for production of IMOs, we developed a simple and efficient method by using enzyme cocktails composed of the recombinant Bacillus naganoensis pullulanase produced by Bacillus licheniformis, a-amylase from Bacillus amyloliquefaciens, barley bran ß-amylase, and a-transglucosidase from Aspergillus niger to perform simultaneous saccharification and transglycosylation to process the liquefied starch. After 13 h of reacting time, 49.09% IMOs (calculated from the total amount of isomaltose, isomaltotriose, and panose) were produced. Conclusions: Our method of using an enzyme cocktail for the efficient production of IMOs offers an attractive alternative to the process presently in use.

PAGES
pp. 46 - 51

 Articles related

Felipe Vasquez-Ponce, Sebastián Higuera-Llantén, María Soledad Pavlov, Ramón Ramírez-Orellana, Sergio H. Marshall, Jorge Olivares-Pacheco    

Background: In recent years, Antarctica has become a key source of biotechnological resources. Native microorganisms have developed a wide range of survival strategies to adapt to the harsh Antarctic environment, including the formation of biofilms. Algi... see more


Hedan Li, Chengwei Hao, Daqing Xu    

Background: Escherichia coli has been widely used as a host to clone and express heterologous genes. However, there are few vectors available for cloning and expressing extremely toxic genes, which limits further basic and applied research on extremely t... see more


Zhiwei Wang,Min Yi,Zheyun Zhang,Cancan Zhu,Qimin Feng,Peng Lu,Shuangfei Wang    

Cellulose paper is the most attractive green packaging material due to its recyclability, renewability, sustainability and biodegradability. In some applications, paper with a high level of water resistance is desirable to meet specific requirements in m... see more


Gordana Manevska-Tasevska, Helena Hansson, Ewa Rabinowicz    

This study estimated the possibilities of reducing costs and improving input use in a sample of Swedish farms specialising in beef production. Input use efficiency was measured in a multi-output, multi-input function distance function analysis, covering ... see more


Oliver Meixner,Sonja Hackl,Rainer Haas    

Palm oil is one of the most important plant oils worldwide and of particular importance for the food industry. In 2017, palm oil accounted for 35 % of the global plant oil production. The area used for palm oil production increased from 6 million hectare... see more