SUMMARY
Background: Hyalodendriella sp. Ponipodef12, an endophytic fungus from a poplar hybrid, was a high producer of
botrallin and TMC-264 with various bioactivities. In this study, the influences of eight metal ions (i.e., Mn2+, Na+,
Mg2+, Zn2+, Cu2+, Fe2+, Fe3+ and Al3+) on botrallin and TMC-264 production in liquid culture of the endophytic
fungus Hyalodendriella sp. Ponipodef12 were investigated.
Results: Three most effective metal ions (Zn2+, Cu2+ and Mg2+) along with their optimum concentrations were
screened. The optimum addition time and concentrations of Zn2+, Cu2+ and Mg2+ were further obtained
respectively for improving botrallin and TMC-264 production. The combination effects of Zn2+, Cu2+ and
Mg2+ on the production of botrallin and TMC-264 by employing statistical method based on the central
composite design (CCD) and response surface methodology (RSM) were evaluated, and two quadratic
predictive models were developed for botrallin and TMC-264 production. The yields of botrallin and TMC-264,
which were predicted as 144.12 mg/L and 36.04 mg/L respectively, were validated to be 146.51 mg/L and
36.63 mg/L accordingly with the optimum concentrations of Zn2+ at 0.81 mmol/L, Cu2+ at 0.20 mmol/L, and
Mg2+ at 0.13 mmol/L in medium.
Conclusion: The results indicated that the enhancement of botrallin and TMC-264 accumulation in liquid culture
of the endophytic fungus Hyalodendriella sp. Ponipodef12 by the metal ions and their combination should be an
effective strategy.