SUMMARY
ABSTRAK. Penelitian dilakukan untuk mengetahui efektifitas ekstrak daun babadotan sebagai bahan aktif cairan pencelup puting (teat dipping) untuk menghambat masuknya bakteri melalui puting, serta membandingkan efektifitasnya dengan povidone iodine. Dua belas ekor sapi perah laktasi penderita mastitis subklinis digunakan dalam rancangan acak lengkap (RAL). Perlakuan berupa teat dipping menggunakan ekstrak daun babadotan 5%, 10%, 15% dan povidone iodine 10% selama 14 hari. Tingkat peradangan kelenjar ambing diketahui dari penjumlahan skor California mastitis test (CMT) dari keempat puting sapi. Hasil penelitian menunjukan penurunan rata-rata total koloni bakteri dalam susu yang sangat signifikan (P<0.01) dari 6,1-7,3 log cfu/ml sebelum pencelupan menjadi 4,4-6,0 log cfu/ml sesudah pencelupan. Tidak ada perbedaan efektifitas sebagai antibakteri antara ekstrak daun babadotan dan povidone iodine dalam menurunkan total koloni bakteri dalam susu. Terjadi penurunan tingkat peradangan kelenjar ambing (P<0.01) dari rata-rata 6,9 sebelum teat dipping menjadi 4,2 sesudah teat dipping. Persentase penurunan tingkat peradangan ambing ekstrak daun babadotan lebih tinggi (P<0.05) dari povidone iodine. pH susu setelah dipping lebih rendah (P<0,05) dari pH susu sebelum dipping. Berdasarkan hasil dapat disimpulkan ekstrak daun babadotan dapat digunakan sebagai alternative antiseptik yang bertindak sebagai anti bakteri dan anti inflamasi dengan konsentrasi terbaik 5% ekstrak. (Effectiveness of Ageratum conyzoides leaves extract as green antiseptic for teat dipping practices in dairy cow) ABSTRACT. The research was conducted to determine the effectiveness babadotan leaf extract (BLE) as an active ingredient of antiseptic teat dip for inhibiting the entry of bacteria through the nipple, and to compare its effectiveness with synthetic antiseptic povidone iodine(PI). Twelve lactating dairycows suffering from subclinical mastitis were used in a completely randomized design. Teat dipping use 5%, 10% and 15% of BLE and PI 10% for 14 days. The result show average total bacterial colonies in milk was reduced significantly (P<0.01) from 6,1-7,3log cfu/ml at before dipping to 4,4-6,0 log cfu/ml after dipping. There are no differences between BLE and PI in reducing total bacterial colonies. A decline in the level of the mammary glands inflammation (P<0.01) from an average at before teat dipping 6.9 to 4.2 after teat dipping. Compared with PI, the percentage decrease in the level of mammary gland’s inflammation of cows using BLE antiseptic is significantly higher (P<0.05). Milk pH at after teat dipping practices is lower (P<0.05) than before dipping. In conclusion, BLE can be used as an alternativeantiseptic due to its effectiveness as antibacterial and anti-inflammatory. The recommendation concentration of the babadotan antiseptic is 5% of extract.