SUMMARY
It is of great importance to maintain the genetic diversity of traditional materials and wild plants. The Emilia applegermplasm was safeguarded, using in vitro tissue culture techniques. Two culture media and six concentrationswere evaluated for disinfection; in addition, eight treatments for budding, using different concentrations of6-benzylaminopurine (BAP) and for rooting four treatments with indole butyric acid (IBA). We used a DCA with a 2x 4 factorial arrangement with six observations. The percentages of contamination, oxidation, viability, days at sprouting,number of shoots and length of shoots were evaluated. Treatments equal to and greater than 10% NaClO for 10minutes had adequate values of non-contamination of explants. The lower number of oxidized explants had a directproportional correlation in the responses to less concentration and time of exposure. The highest percentage of viableexplants (77%) was expressed in 10% NaClO treatment for 15 minutes. The best responses regarding the variabledays to budding were presented in the treatments without BAP intervention. The best response in the number of outbreakswas manifested for the 1 ppm BAP treatment in the culture media (Murashige and Skoog modified - MSM andModified CHU 10 by Gerloff). The best response in the size of the outbreak was expressed in the treatment CHU + 1ppm BAP. The cellular tissues the Emilia apple show high susceptibility to microbial contaminants and the oxidationof tissues, which makes it difficult to multiply by in vitro culture. High concentrations of BAP inhibit spontaneousphysiological responses in explants of the Emilia apple, especially in the early stages where there is greater productionof intrinsic hormonal effects.