SUMMARY
Rikalo N. A. ????????? ???????????? ??????????? ??????? ??????? ? ??????????????? ????? ??? ?????????? ?????????? ???????? = The mechanisms of reparative regeneration of liver tissue in immature rats at chronic toxic hepatitis. Journal of Education, Health and Sport. 2015;5(9):587-598. ISSN 2391-8306. DOI10.5281/zenodo.31627http://dx.doi.org/10.5281/zenodo.31627http://ojs.ukw.edu.pl/index.php/johs/article/view/2015%3B5%289%29%3A587-598https://pbn.nauka.gov.pl/works/639447Formerly Journal of Health Sciences. ISSN 1429-9623 / 2300-665X. Archives 2011–2014http://journal.rsw.edu.pl/index.php/JHS/issue/archive Deklaracja.Specyfika i zawartosc merytoryczna czasopisma nie ulega zmianie.Zgodnie z informacja MNiSW z dnia 2 czerwca 2014 r., ze w roku 2014 nie bedzie przeprowadzana ocena czasopism naukowych; czasopismo o zmienionym tytule otrzymuje tyle samo punktów co na wykazie czasopism naukowych z dnia 31 grudnia 2014 r.The journal has had 5 points in Ministry of Science and Higher Education of Poland parametric evaluation. Part B item 1089. (31.12.2014).© The Author (s) 2015;This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland and Radom University in Radom, PolandOpen Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License(http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non commercialuse, distribution and reproduction in any medium, provided the work is properly cited.The authors declare that there is no conflict of interests regarding the publication of this paper.Received: 02.08.2015. Revised 05.09.2015. Accepted: 29.09.2015. ???: 591.436:599.323.4:616.36-002 ????????? ???????????? ??????????? ??????? ??????? ? ??????????????? ????? ??? ?????????? ?????????? ????????THE MECHANISMS OF REPARATIVE REGENERATION OF LIVER TISSUE IN IMMATURE RATS AT CHRONIC TOXIC HEPATITIS ?. ?. ??????N. A. Rikalo ?????????? ???????????? ???????? ??????????? ????? ?.?. ????????Vinnitsia National Pirogov memorial Medical UniversityAbstractIn experiments on immature white laboratory male rats investigated mechanisms of reparative regeneration of liver tissue by flow cytometry on the model of chronic toxic hepatitis induced by intragastric administration CCl4 and 5% ethanol solution for 60 days.The number of nuclei in phases G0-G1 of cell cycle decreases, resulting from highly alteration of hepatocytes, which are the most sensitive to the injury and also as a result of mobilization of reserves (G0 phase) for regeneration were established. The percent of nuclei in S- phase was higher by 1.5 times, which proves activation of DNA synthesis in response to alteration. This testified about compensatory activation of reparative regeneration of liver cells. Alteration of liver parenchyma by hepatotoxins that manifests by processes of necroapoptosis and simultaneously activation of the cell cycle, transformation of G0 phase to phase G1, S, G2M, indicates the inclusion of protective mechanisms of reparative regeneration.The characteristic feature of the population of immature hepatocytes of rats with experimental chronic toxic hepatitis is an increase in nuclear DNA ploidy more than 8c (in 2,2 times, p<0,01), the decrease in the percentage of nuclei in the range G0/G1 (in 7,1%, p<0,01), an increase - in the S-phase (in 58,3%, p<0,05) increase in proliferation index (in 23,0%, p<0,01) and fragmentation of nuclear DNA (in 2 times, p<0,001). On the results of experimental studies proved that reparative regeneration of the liver tissue of immature rats at ethanol and CCl4 chronic injury develops by the mechanism of polyploidy hepatocytes that are more resistant to damaging factors, but do not perform specialized functions.Key words: chronic toxic hepatitis, reparative regeneration, hepatocytes. ??????. ? ???????? ?? ??????????????? ????? ???????????? ?????-?????? ???????????? ????????? ???????????? ??????????? ??????? ??????? ??????? ????????? ?????????? ?? ?????? ?????????? ?????????? ????????, ???????????? ???????????????? ????????? CCl4 ?? 5% ??????? ??????? ???????? 60 ????.???????????, ?? ????????? ???? ??????, ??? ?????????? ? ????? G0-G1, ???? ?????????? ??????, ?? ???? ???? ????????? ?????????? ????????????????????? ???????????, ??? ? ???????? ????????? ?? ??????????, ? ????? ??????????? ???????? (G0 ????) ??? ???????????? ???????????. ???????? ???? ? S-???? ??? ??????? ? 1,5 ???? (?<0,05), ?? ???????? ????????? ??????? ??? ? ????????? ?? ??????????, ????? ???????????? ????????????? ????????? ???????? ???????????? ??????????? ?????? ???????. ?????????? ????????? ??????? ???????????????, ??? ???????????? ????????? ????????????? ? ????????? ??????? ????????? ????, ????? ??????? ?????? ? ???? ?????? (G0) ? ???? G1, S, G2M, ???????? ??? ????????? ???????? ?????????? ???????????? ???????????.??????????? ??????? ????????? ??????????? ??????????????? ????? ??? ?????????????????? ?????????? ?????????? ???????? ? ?????????? ?????????? ??????? ??? >8? (? 2,2 ????, ?<0,01), ????????? ???????? ???? ? ????????? G0/G1 (?? 7,1 %, ?<0,01), ?????????? – ? S-???? (?? 58,3 %, ?<0,05), ?????????? ??????? ???????????? (?? 23,0 %, ?<0,01) ?? ???????????? ??????? ??? (? 2 ????, ?<0,001). ?? ???????????? ??????????? ?????????????????? ??????????? ????????, ?? ??????????? ??????????? ??????? ??????? ??????????????? ????? ??? ?????????? ?????????? ??l4 ? ????????? ? ????????, ???????????? ?? ?????????? ??????????? ???????????, ??? ????? ?????? ?? ??? ?????????? ????????, ??? ?? ?????? ?????? ????? ?????????? ?????????????? ???????.??????? ?????: ????????? ????????? ???????, ??????????? ???????????, ??????????.