SUMMARY
everse-phase chromatography, linear gradient and multichannel UV-detecting substances. The method allows obtaining reliable results of studies of drugs and mixtures in biological objects.The regression coefficients of calibration curve were calculated by the method of least squares with the equation of the line S = 0.00134 ?. Correlation coefficient equaled 0.9993. Validation characteristics of HPLC-method for determination of prazosin hydrochloride: linearity range (10.0–200.0 µg / ml), limit of quantitative determination (10.0 µg / ml or 40 ng of sample), correctness and accuracy, which based on the quantitative determination results of the preparation by HPLC method in the model solutions (RSD=67.9 %) were calculated. It is established that the relative uncertainty of the average result was not exceeded +1.89 % when using the proposed method of HPLC analysis of prazosin hydrochloride in model solutions.