SUMMARY
The 3-hydroxy-3-methyl-glutaryl-coenzyme A (HMG-CoA) reductase is the key enzyme of cholesterol biosynthesis pathway in the liver. The present work aimed at investigating the hypolipidemic effect of total extract from Pandanus amaryllifolius Roxb. (PA) leaves through the inhibition of HMG-CoA reductase activity. PA extract showed a moderate in vitro inhibitory effect on activity of HMG-CoA reductase with IC50 value at range of 126.42 microg/ml as compared to that of atorvastatin about 7.77 ng/ml. In a mouse model of hyperlipidemia induced by a single intravenous injection of triton WR-1339, oral administration of PA extract significantly reduced total cholesterol as well as triglyceride levels as compared to hyperlipidemic mice. Furthermore, PA extract also inhibited HMG-CoA reductase activity with reduction of 32%, which were comparable to those of positive control, atorvastatin. In conclusion, PA extract clearly showed promising hypolipidemic effect by inhibiting HMG-CoA reductase activity.