SUMMARY
The present disease biomarker limits from low diagnostic sensitivity, specificity and have not yet made a major input on reducing disease burden. Because of the complex nature of biological fluids such as plasma biomarker discovery efforts using proteomics have not yet delivered any novel marker. Recently so many cell culture based model of the disease to identify novel candidate of disease biomarker. Cell secreted protein to serve as serological marker, play a central role in pathophysiology of the disease. Research has recognized numerous cellular events and mediators coupled with wound healing that can serve as biomarkers. In-vitro macrophagic cell culture study laid down first time with reference to assess wound healing activity. Macrophages play a key role in all stages of wound healing. In the present work an attempt was made to study effect of hydrogel containing rutin in different concentration on various haematological parameters and on the basis of above finding the macrophagic cell culture investigation was laid down. The present work showed the effect of different rutin concentrations (0.20µM, 0.25 µM & 0.30 µM) on macrophage cell culture and the result illustrated that 0.025 µM concentration drug significantly increased growth of macrophage in cell culture. Thus, it promoted wound healing activity.