ARTICLE
TITLE

Determination of diphenhydramine by HPLC method in biological liquids

SUMMARY

HPLC analysis was carried out on a microcolumn liquid chromatograph "Milichrome A-02" in conditions: reversed-phase variant, column with non-polar sorbent Prontosil 120-5 C18 AQ, 5 µm; mobile phase in the mode of linear gradient – from eluent ? (5 % acetonitrile and 95 % buffer solution) to eluent B (100 % acetonitrile) as during 40 min. The flow rate of the mobile phase has been formed 100 µl/min, injection volume – 4 µl. Multichannel detection of the substance was carried out using a UV spectrophotometer at 210, 220, 230, 240, 250, 260, 280 and 300 nm; the optimal value of column temperature – 37 - 40°? and pressure of pump – 2.8 – 3.2 MPa.

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