SUMMARY
Objective: To determine the in vitro antiviral activity of different extracts from Dodonaea viscosa leaves against coxackievirus B3 (CVB3) and rotavirus SA-11 (RV SA-11) infections.Methods: The cytotoxic effect of the extracts on GMK and MA 104 cells was determined by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) reduction colorimetric assay. To evaluate the antiviral effect of the plant extracts, three different strategies were used.Results: D. viscosa exhibited ttherapeutic index (TI) ranging from 0.3 to 25 with reduction in virus titre ranging from 0.25 to 5 log10 TCID50/0.1 ml for CVB3 whereas TI ranging from 0.4 to 29.2 with reduction in virus titre ranging from 0.25 to 5.25 log10 TCID50 for RV SA-11. Crude extract provided the potent inhibition of CVB3 and RV SA-11 replication by binding to a viral capsid of CVB3 and viral receptor of RV SA-11 preventing viruses entry into host cells for both viruses.Conclusions: We conclude that the crude extract of D. viscosa leaves shows the potential antiviral effect to CVB3 and RV SA-11 infections.