Journal title
Journal of Nematology

   
ARTICLE
TITLE

Genetic variation of Hoplolaimus columbus populations in the United States using PCR-RFLP analysis of nuclear rDNA ITS regions

SUMMARY

Hoplolaimus columbus is an important nematode pest which causes economic loss of crops including corn, cotton, and soybean in the Southeastern United States. DNA sequences of the ITS1-5.8S-ITS2 region of ribosomal DNA from H. columbus were aligned and analyzed to characterize intraspecific genetic variation between eleven populations collected from Georgia, Louisiana, North Carolina, and South Carolina. In comparative sequence analysis with clones from either one or two individuals obtained from the eleven populations, we found variability existed among clones from an individual and that clonal diversity observed from within individuals was verified by PCR-RFLP. PCR-RFLP analysis with Rsa I and Msp I restriction enzymes yielded several fragments on 3.0% agarose gel that corresponded to different haplotypes in all populations and the sum of digested products exceeded the length of undigested PCR products, which revealed that ITS heterogeneity existed in a genome of H. columbus. This indicates that heterogeneity may play a role in the evolution of this parthenogenetic species.

PAGES
pp. 187 - 193

 Articles related

P. W. Hunt, J. McNally, W. Barris, M. L. Blaxter    

In common with many other groups, nematodes express globins with unknown functions. Nematode globin-like genes can be divided into class 1 globins, similar to vertebrate myoglobins, and a wide range of additional classes. Here we show that class 1 nemato... see more


C. H. Bae, A. L. Szalanski, R. T. Robbins    

DNA sequence analyses of the nuclear ribosomal ITS1 region of the ribosomal DNA and D1-D3 expansion segments of the 28S gene were conducted to characterize the genetic variation of six amphimictic Hoplolaimus species, including H. magnistylus, H. concaud... see more


S. Anaid Diaz, Jan Lindstrom, Daniel T. Haydon    

Species of the Caenorhabditis genus have been used as model systems in genetics and molecular research for more than 30 years. Despite this, basic information about their demography, in the wild and in the lab, has remained unknown until very recently. H... see more


E. T. Cofcewicz, R. M. D. G. Carneiro, O. Randig, C. Chabrier, P. Queneherve    

Ninety-six isolates of Meloidogyne species collected from banana fields from Martinique, Guadeloupe, and French Guiana, were examined using esterase (Est) and malate dehydrogenase (Mdh) phenotypes. Adult females identified as M. arenaria, M. incognita, M... see more


T. O. Powers, P. G. Mullin, T. S. Harris, L. A. Sutton, R. S. Higgins    

A regional nematode survey of potato fields was conducted in the central United States during 2002 and 2003. The survey encompassed seven states and included a morphological and molecular examination of nematodes of regulatory concern from 1,929 soil sam... see more