Genetic Diversity of Elaeagnus angustifolia L. (Elaeagnaceae) Populations in İzmir (Turkey)

  • Erengül SOFYALIOĞLU Faculty of Agriculture, Adnan Menderes University, South Campus, Cakmar, Aydin
  • Emre SEVİNDİK Faculty of Agriculture, Adnan Menderes University, South Campus, Cakmar, Aydin
  • Hüseyin UYSAL Faculty of Agriculture, Adnan Menderes University, South Campus, Cakmar, Aydin
Keywords: Elaeagnus angustifolia, ISSR, genetic diversity, Turkey.

Abstract

This study was performed out genetic diversity of some Elaeagnus angustifolia L. populations growing in İzmir province by using ISSR markers. In the study, PCR was performed using 15 ISSR primers. PCR products were run in agarose gel and visualized under UV light. Amplified products were scored as follows. A total of 46 bands were produced from 15 ISSR primers, of which 27 were polymorphic. The proportion of polymorphic bands was evaluated as approximately 58.7%. Genetic distances between phylogenetic trees and genotypes were calculated using the PAUP program. The phylogenetic tree consists of two large clades. The longest distance between populations was between Gümüldür-Özdere and Çeşme-Alaçatı population with a value of 0.50, while the closest distance was between Çeşme-Ayayorgi and Konak-Hatay populations with a value of 0.06. The results show that ISSR markers are useful tools for determining genetic relationships between E. angustifolia populations

Author Biographies

Erengül SOFYALIOĞLU, Faculty of Agriculture, Adnan Menderes University, South Campus, Cakmar, Aydin
Department of Agricultural Biotechnology
Emre SEVİNDİK, Faculty of Agriculture, Adnan Menderes University, South Campus, Cakmar, Aydin
Department of Agricultural Biotechnology
Hüseyin UYSAL, Faculty of Agriculture, Adnan Menderes University, South Campus, Cakmar, Aydin
Department of Agricultural Biotechnology
Published
2021-05-14