Morphological and Physiological Development of Pyricularia oryzae Isolates from North-western Region of Sarawak on Different Media under Laboratory Conditions

Growth of Pyricularia oryzae on different media

  • ELISSA STELLA RAFAEL Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300, Kota Samarahan, Sarawak, Malaysia
  • YEO FREDDY KUOK SAN Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300, Kota Samarahan, Sarawak, Malaysia
  • NOR AIN HUSSIN Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300, Kota Samarahan, Sarawak, Malaysia
  • VU THANH TU ANH Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300, Kota Samarahan, Sarawak, Malaysia
  • ANG POH SIM Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300, Kota Samarahan, Sarawak, Malaysia
  • EWE ZHANG HUA Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300, Kota Samarahan, Sarawak, Malaysia
  • LAI LEE SAN Agriculture Research Centre Semongok, Department of Agriculture Sarawak, KM20, Borneo Heights Road, 93250 Kuching, Sarawak Malaysia
DOI: https://doi.org/10.33736/bjrst.5258.2023
Keywords: Growth media, Pyricularia oryzae, rice blast, Sarawak, sporulation

Abstract

Rice blast (causal agent: Pyricularia oryzae) is an important disease of rice in Sarawak. Understanding the pathogen’s morphological characteristics, genetic diversity and pathogenicity is important. Having a suitable medium for culturing and maintaining P. oryzae is important to ensure the availability of inoculum or materials under laboratory conditions. Oatmeal agar (OMA) and potato dextrose agar (PDA) are common media used for growing P. oryzae. OMA allows better mycelial growth and better sporulation as compared to PDA. There are also other alternatives such as fresh rice leaf agar and rice straw agar. Although OMA seems to be the best medium, unfortunately the opaqueness of the medium causes difficulty in observing the morphology and growth of mycelia. In addition, it is known that different isolates of P. oryzae will respond differently to different media. This study aims to identify the best media for culturing and maintaining P. oryzae isolates from Sarawak. A total of 14 P. oryzae isolates were characterised for their morphological characteristics, growth rate and sporulation rate using seven growing media. These 14 isolates included seven newly identified isolates in this study and seven isolates from a previous study, which were verified using internal transcribed spacer DNA sequence. The colony surface of the 14 P. oryzae isolates varied on different growing media. The pigmentation of colony surface varied from different shades of grey, translucent light brown, white and colourless. Pyricularia oryzae isolates grew better on OMA and PDA, while OMA was the best for sporulation. These two media can be recommended for culturing and maintaining different P. oryzae isolates under laboratory conditions.

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Published
2023-06-30
How to Cite
ELISSA STELLA RAFAEL, YEO FREDDY KUOK SAN, NOR AIN HUSSIN, VU THANH TU ANH, ANG POH SIM, EWE ZHANG HUA, & LAI LEE SAN. (2023). Morphological and Physiological Development of Pyricularia oryzae Isolates from North-western Region of Sarawak on Different Media under Laboratory Conditions . Borneo Journal of Resource Science and Technology, 13(1), 81-92. https://doi.org/10.33736/bjrst.5258.2023
Issue
Vol 13 No 1 (2023): Borneo Journal of Resource Science and Technology
Section
General 2

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