Berberine triggers necroptosis of cervical H8 cells by activating receptor-interacting protein kinase 1

Authors

  • Mengting Wang Department of Obstetrics and Gynecology, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, Jiangsu 215101, China.
  • Huixian Chen Department of Obstetrics and Gynecology, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, Jiangsu 215101, China. https://orcid.org/0009-0001-7684-0888
  • Qian Wu Department of Critical Care Medicine, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, Jiangsu 215101, China. https://orcid.org/0009-0002-8060-1393
  • Yan Huang Department of Obstetrics and Gynecology, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, Jiangsu 215101, China. https://orcid.org/0000-0003-4801-3444
  • Yuzhen Zhou Department of Obstetrics and Gynecology, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, Jiangsu 215101, China. https://orcid.org/0000-0002-6978-3432

DOI:

https://doi.org/10.3329/bjp.v19i1.70889

Keywords:

Berberine, cervical H8 Cells, Cervical intraepithelial neoplasia, Necroptosis, Receptor-interacting protein kinase 1, Western blot

Abstract

This study aims to investigate the effect of berberine on necroptosis in cervical H8 cells. CCK-8 assay was used to assess cell proliferation activity. The expression levels of necroptosis key markers Receptor-interacting protein kinase 1 (RIPK1), MLKL, p-MLKL, and apoptosis-related caspase-8, caspase-3 were analyzed through qRT-PCR, western blot, and immunofluorescence. Mechanistic studies were conducted using the RIPK1 kinase inhibitor necrostatin-1 (Nec-1). The results showed that berberine could inhibit H8 cell proliferation in a time- and dose-dependent manner (p<0.05). It increased the expression of RIPK1 and MLKL (p<0.05), while inhibiting the expression of caspase-8 and caspase-3 (p<0.05). Nec-1 inhibitor decreased the expression of RIPK1 and MLKL after the intervention of berberine (p<0.05). Therefore, berberine induces cervical H8 cell death through the activation of RIPK1-mediated necroptosis.

Downloads

Download data is not yet available.
Abstract
69
Download
99

References

Athanasiou A, Veroniki AA, Efthimiou O, Kalliala I, Naci H, Bowden S, Paraskevaidi M, Arbyn M, Lyons D, Martin-Hirsch P, Bennett P, Paraskevaidis E, Salanti G, Kyrgiou M. Comparative effectiveness and risk of preterm birth of local treatments for cervical intraepithelial neoplasia and stage IA1 cervical cancer: A systematic review and network meta-analysis. Lancet Oncol. 2022; 23: 1097-108.

Bertheloot D, Latz E, Franklin BS. Necroptosis, pyroptosis and apoptosis: An intricate game of cell death. Cell Mol Immunol. 2021; 18: 1106-21.

Chen X, Hu X, Liu L, Liang X, Xiao J. Extracts derived from a traditional Chinese herbal formula triggers necroptosis in ectocervical Ect1/E6E7 cells through activation of RIP1 kinase. J Ethnopharmacol. 2019; 239: 111922.

Doorbar J, Egawa N, Griffin H, Kranjec C, Murakami I. Human papillomavirus molecular biology and disease association. Rev Med Virol. 2015; 25 Suppl 1: 2-23.

Feng X, Song Q, Yu A, Tang H, Peng Z, Wang X. Receptor-interacting protein kinase 3 is a predictor of survival and plays a tumor suppressive role in colorectal cancer. Neoplasma 2015; 62: 592-601.

Feoktistova M, Leverkus M. Programmed necrosis and necroptosis signalling. Febs J. 2015; 282: 19-31.

Floriano BF, Carvalho T, Lopes TZ, Takahashi L, Rahal P, Tedesco AC, Calmon MF. Effect of berberine nanoemulsion Photodynamic therapy on cervical carcinoma cell line. Photodiagnosis Photodyn Ther. 2021; 33: 102174.

Gao W, Wang X, Zhou Y, Wang X, Yu Y. Autophagy, ferrop-tosis, pyroptosis, and necroptosis in tumor immunotherapy. Signal Transduct Target Ther. 2022; 7: 196.

Ghaem-Maghami S, Sagi S, Majeed G, Soutter WP. Incomplete excision of cervical intraepithelial neoplasia and risk of treatment failure: A meta-analysis. Lancet Oncol. 2007; 8: 985-93.

Hoffman SR, Le T, Lockhart A, Sanusi A, Dal Santo L, Davis M, McKinney DA, Brown M, Poole C, Willame C, Smith JS. Patterns of persistent HPV infection after treatment for cervical intraepithelial neoplasia (CIN): A systematic review. Int J Cancer. 2017; 141: 8-23.

Jie H, He Y, Huang X, Zhou Q, Han Y, Li X, Bai Y, Sun E. Necrostatin-1 enhances the resolution of inflammation by specifically inducing neutrophil apoptosis. Oncotarget 2016; 7: 19367-81.

Kaczmarek A, Vandenabeele P, Krysko DV. Necroptosis: The release of damage-associated molecular patterns and its physiological relevance. Immunity 2013; 38: 209-23.

Kim JH, Weeratunga P, Kim MS, Nikapitiya C, Lee BH, Uddin MB, Kim TH, Yoon JE, Park C, Ma JY, Kim H, Lee JS. Inhibitory effects of an aqueous extract from Cortex Phellodendri on the growth and replication of broad-spectrum of viruses in vitro and in vivo. BMC Complement Altern Med 2016; 16: 265.

Li Q, Zhao H, Chen W, Huang P. Berberine induces apoptosis and arrests the cell cycle in multiple cancer cell lines. Arch Med Sci. 2023; 19: 1530-37.

Liontos M, Kyriazoglou A, Dimitriadis I, Dimopoulos MA, Bamias A. Systemic therapy in cervical cancer: 30 years in review. Crit Rev Oncol Hematol. 2019; 137: 9-17.

Lim HS, Moon BC, Park G. Nrf2 signaling contributes to the neuroprotective effect of Cicadidae Periostracum against 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine toxicity. Bangladesh J Pharmacol. 2022; 17: 1-8.

Liu L, Fan J, Ai G, Liu J, Luo N, Li C, Cheng Z. Berberine in combination with cisplatin induces necroptosis and apoptosis in ovarian cancer cells. Biol Res. 2019; 52: 37.

Mahata S, Bharti AC, Shukla S, Tyagi A, Husain SA, Das BC. Berberine modulates AP-1 activity to suppress HPV transcription and downstream signaling to induce growth arrest and apoptosis in cervical cancer cells. Mol Cancer. 2011; 10: 39.

Mantena SK, Sharma SD, Katiyar SK. Berberine, a natural product, induces G1-phase cell cycle arrest and caspase-3-dependent apoptosis in human prostate carcinoma cells. Mol Cancer Ther. 2006; 5: 296-308.

N KE, Koutras A, Syllaios A, Schizas D, Kalagasidou S, Pagkalos A, Alatzidou D, Kantari P, Ntounis T, Fasoulakis Z. Basic principles of molecular biology of cancer cell: Molecular cancer indicators. J Buon. 2021; 26: 1723-34.

Park HH, Kim HR, Park SY, Hwang SM, Hong SM, Park S, Kang HC, Morgan MJ, Cha JH, Lee D, Roe JS, Kim YS. RIPK3 activation induces TRIM28 derepression in cancer cells and enhances the anti-tumor microenvironment. Mol Cancer. 2021; 20: 107.

Park SY, Park HH, Park SY, Hong SM, Yoon S, Morgan MJ, Kim YS. Reduction in MLKL-mediated endosomal trafficking enhances the TRAIL-DR4/5 signal to increase cancer cell death. Cell Death Dis. 2020; 11: 744.

Ruan J, Mei L, Zhu Q, Shi G, Wang H. Mixed lineage kinase domain-like protein is a prognostic biomarker for cervical squamous cell cancer. Int J Clin Exp Pathol. 2015; 8: 15035-38.

Rui Z, Chang-Pei X, Jing-Jing Z, Hong-Jun Y. [Research progress on chemical compositions of Coptidis Rhizoma and pharmacological effects of berberine]. Zhongguo Zhong Yao Za Zhi. 2020; 45: 4561-73.

Sawai H. Induction of apoptosis in TNF-treated L929 cells in the presence of necrostatin-1. Int J Mol Sci. 2016; 17: 1678.

Shahsavari Z, Karami-Tehrani F, Salami S, Ghasemzadeh M. RIP1K and RIP3K provoked by shikonin induce cell cycle arrest in the triple negative breast cancer cell line, MDA-MB-468: Necroptosis as a desperate programmed suicide pathway. Tumour Biol. 2016; 37: 4479-91.

Song BB, Xu MY, Xu XF, Li X, Chen M. Zoledronic acid inhibits the bone metastasis of breast cancer by repressing exosome-loaded DKK1. Bangladesh J Pharmacol. 2022; 17: 92-101.

Sun W, Yu W, Shen L, Huang T. MLKL is a potential prognostic marker in gastric cancer. Oncol Lett. 2019; 18: 3830-36.

Trope A, Sjoborg K, Eskild A, Cuschieri K, Eriksen T, Thoresen S, et al. Performance of human papillomavirus DNA and mRNA testing strategies for women with and without cervical neoplasia. J Clin Microbiol. 2009; 47: 2458-64.

Vanden BT, Linkermann A, Jouan-Lanhouet S, Walczak H, Vandenabeele P. Regulated necrosis: The expanding net-work of non-apoptotic cell death pathways. Nat Rev Mol Cell Biol. 2014; 15: 135-47.

Verdonck S, Nemegeer J, Vandenabeele P, Maelfait J. Viral manipulation of host cell necroptosis and pyroptosis. Trends Microbiol. 2022; 30: 593-605.

Warowicka A, Nawrot R, Gozdzicka-Jozefiak A. Antiviral activity of berberine. Arch Virol. 2020; 165: 1935-45.

Zhang G, Wang J, Zhao Z, Xin T, Fan X, Shen Q, Raheem A, Lee CR, Jiang H, Ding J. Regulated necrosis, a proinflammatory cell death, potentially counteracts pathogenic infections. Cell Death Dis. 2022; 13: 637.

Zhu M, He Y, Baak JP, Zhou X, Qu Y, Sui L, Feng W, Wang Q. Factors that influence persistence or recurrence of high-grade squamous intraepithelial lesion with positive margins after the loop electrosurgical excision procedure: A retros-pective study. BMC Cancer. 2015; 15: 744.

Downloads

Published

2024-03-14

How to Cite

Wang, M., H. Chen, Q. Wu, Y. . Huang, and Y. . Zhou. “Berberine Triggers Necroptosis of Cervical H8 Cells by Activating Receptor-Interacting Protein Kinase 1”. Bangladesh Journal of Pharmacology, vol. 19, no. 1, Mar. 2024, pp. 13-22, doi:10.3329/bjp.v19i1.70889.

Issue

Vol. 19 No. 1 (2024)

Section

Research Articles

License

Copyright (c) 2024 Mengting Wang, Huixian Chen, Qian Wu, Yan Huang, Yuzhen Zhou

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).