Resveratrol Limits Lipogenesis and Enhance Mitochondrial Activity in HepG2 Cells

Authors

  • Magdalena Izdebska Department of Toxicology, Medical University of Lublin, Lublin, Poland. http://orcid.org/0000-0001-5999-4300
  • Mariola Herbet Department of Toxicology, Medical University of Lublin, Lublin, Poland.
  • Monika Gawrońska-Grzywacz Department of Toxicology, Medical University of Lublin, Lublin, Poland.
  • Iwona Piątkowska-Chmiel Department of Toxicology, Medical University of Lublin, Lublin, Poland.
  • Agnieszka Korga Independent Medical Biology Unit, Medical University of Lublin, Lublin, Poland.
  • Marcin Sysa Department of Toxicology, Medical University of Lublin, Lublin, Poland.
  • Magdalena Iwan Independent Medical Biology Unit, Medical University of Lublin, Lublin, Poland.
  • Dorota Natorska-Chomicka Department of Toxicology, Medical University of Lublin, Lublin, Poland.
  • Ewa Poleszak Department of Applied Pharmacy, Medical University of Lublin, Lublin, Poland.
  • Andrzej Wróbel Second Department of Gynecology, Medical University of Lublin, Lublin, Poland.
  • Sławomir Mandziuk Department of Pneumology, Oncology and Allergology, Medical University of Lublin, Lublin, Poland.
  • Jarosław Dudka Department of Toxicology, Medical University of Lublin, Lublin, Poland.

DOI:

https://doi.org/10.18433/jpps29994

Abstract

Purpose: The aim of this study was to evaluate the effect of resveratrol on de novo lipogenesis in HepG2 cells caused by high glucose concentrations. Increased lipogenesis in the liver is the main reason for the development of nonalcoholic fatty liver disease (NAFLD) - currently one of the most common chronic liver diseases. In developed countries, this disease is mostly associated with nutritional disorders, resulting from the increasing consumption of monosaccharides. Resveratrol is a natural polyphenol with a promising potential for NAFLD treatment. Methods: The steatosis of HepG2 cells was visualized using the intracellular lipid staining by Nile Red dye with a fluorescence microscope. This study also evaluated the effect of resveratrol on the mitochondrial activity (MitoTracker Green staining), dsDNA (Hoechst 33342 staining) and the viability of HepG2 cells treated with high glucose concentrations (25 and 33 mM). Results: Current study showed that high glucose concentrations induced fat-overloading in HepG2 cells (microvacuolar steatosis occurred in most of the cells). Resveratrol (20 μM) limits the steatosis induction in HepG2 cells by glucose and increased the mitochondrial activity of cells. Resveratrol did not affect the viability of HepG2 cells. Conclusion: This beneficial effect could be helpful in the treatment of NAFLD.

Downloads

Download data is not yet available.

Author Biography

Magdalena Izdebska, Department of Toxicology, Medical University of Lublin, Lublin, Poland.

Department of Toxicology

Downloads

Published

2018-12-07

How to Cite

Izdebska, M., Herbet, M., Gawrońska-Grzywacz, M., Piątkowska-Chmiel, I., Korga, A., Sysa, M., … Dudka, J. (2018). Resveratrol Limits Lipogenesis and Enhance Mitochondrial Activity in HepG2 Cells. Journal of Pharmacy & Pharmaceutical Sciences, 21(1), 504–515. https://doi.org/10.18433/jpps29994

Issue

Vol. 21 (2018): Pages 305 - 515

Section

Pharmaceutical Sciences; Original Research Articles

License

This is an open access journal with free of charge non-commercial download. At the time of submission, authors will be asked to transfer the copyright to the accepted article to the Journal of Pharmacy and Pharmaceutical Sciences. The author may purchase the copyright for $500 upon which he/she will have the exclusive copyright to the article. Nevertheless, acceptance of a manuscript for publication in the Journal is with the authors' approval of the terms and conditions of the Creative Commons copyright license Creative Common license (Attribution-ShareAlike) License for non-commercial uses.

CLOCKSS system has permission to collect, preserve, and serve this Archival Unit.