Carotenoids, Tocopherols and Antioxidant Activity of Lipophilic Extracts from Sea Buckthorn Berries (Hippophae rhamnoides), Apricot Pulp and Apricot Kernel (Prunus armeniaca)
Abstract
A healthy human diet requires the daily consumption of fruits and vegetables rich in bioactive compounds. Sea buckthorn berries (Hippophae rhamnoides L.) and apricot fruits (Prunus armeniaca L.) are cultivated and appreciated in Romania both as fresh fruits and as derived products. Characterized by a complex chemical composition, sea buckthorn is rich in unsaturated lipids, carotenoids and tocopherols. Except for β-carotene content, less is known about other lipophilic compounds in apricot fruits.
The aim of this paper was to separate and quantify the individual carotenoids, tocopherols and tocotrienols in sea buckthorn, apricot pulp and kernels and also to determine the antioxidant activity of the lipophilic extracts using the TEAC method. Chemical characterization of lipophilic extract was performed by HPLC with PDA and fluorescence detection.
The total carotenoid content was 17.19±1.4 mg/100g F.W. in sea buckthorn; 3.51±0.25 mg/100g F.W. in apricot fruits and 0.58±0.04 mg/100 g F.W. in apricot kernels. The major carotenoids in sea buckthorn were β-carotene, zeaxanthin and β-cryptoxanthin esters. Apricots fruits are rich in β-carotene and its geometric isomers while in kernels we could properly identified only lycopene. The α-tocopherol concentration was higher in sea buckthorn (46 mg/kg) than in apricot fruits (1.09 mg/kg) while apricot kernel contain large amounts of γ-tocopherol (111 mg/kg). Sea buckthorn fruits showed the highest antioxidant capacity, correlated with a high content of both tocopherols and carotenoids.
References
Andersson SC, Olsson ME, Johansson E, Rumpunen K (2009). Carotenoids in Sea Buckthorn (Hippophae rhamnoides L.) Berries during Ripening and Use of Pheophytin a as a Maturity Marker, J. Agric. Food Chem., 57(1):250–258.
Apak R, Gorinstein S, Böhm V, Schaich K, Özyürek M, Güçlü K, (2013). Methods of measurement and evaluation of natural antioxidant capacity/activity (IUPAC Technical report)*, Pure Appl. Chem., 85(5):957-998.
Bal LM, Meda V, Naik SN, Santosh S (2011). Sea buckthorn berries: A potential source of valuable nutrients for nutraceuticals and cosmoceuticals, Food Res.Int., 44: 1718–1727.
Breithaupt DE, Schwack W (2000). Determination of free and bound carotenoids in paprika (Capsicum annuum L.) by LC/MS. Eur Food Res Technol 211: 52–55.
Britton G, Liaaen S, Pfander HP (1995A). Carotenoids, Vol.1A: Isolation and Analysis, Birkhauser Verlag, Basel.
Britton G, Liaaen-Jensen S, Pfander HP (1995B). Carotenoids, Volume 1B: Spectroscopy, Birkhäuser Verlag, Basel, Boston, Berlin.
Chen C, Xu X-M, Chen Y, Yu M-Y, Wen F-Y, Zhang H (2013). Identification, quantification and antioxidant activity of acylated flavonol glycosides from sea buckthorn (Hippophae rhamnoides ssp. sinensis), Food Chemistry,141:1573–1579.
Dragovic-Uzelac V, Levaj B, Mrkic V, Bursac D, Boras M (2007). The content of polyphenols and carotenoids in three apricot cultivars depending on stage of maturity and geographical region.Food Chemistry, 102:966–975.
Etsuo N (2010). Assessment of Antioxidant Capacityin vitro and in vivo, Free Radical Biology & Medicine 49, 503–515, DPPH assay and peroxyl radical scavenging assay, Food Chemistry, 129: 139–148.
Erdogan-Orhan I, Kartal M (2011). Insights into research on phytochemistry and biological activities of Prunus armeniaca L. (apricot), Food Research International, 1238-1243.
Gao X, Ohlander M, Jeppsson N, Trajkovski V (2000). Changes in Antioxidant Effects and Their Relationship to Phytonutrients in Fruits of Sea Buckthorn (Hippophae rhamnoides L.) during Maturation, . Agric. Food Chem.,48 (5):1485–1490.
Gayosso-GarcÃa Sancho L, Elhadi MY, Gustavo AG Aguilar (2013). Contribution of Major Hydrophilic and Lipophilic Antioxidants from Papaya Fruit to Total Antioxidant Capacity, Food and Nutrition Sciences, 4:93-100.
Giuffrida D, Pintea A, Dugo P, Torre G, Pop RM, Mondello L (2012). Determination of carotenoids and their esters in fruits of sea buckthorn (Hippophae rhamnoides L.) by HPLC-DAD-APCI-MS, Phytochemical Analysis, 23:267-273.
Gliszczynska-Swigło A (2006). Antioxidant activity of water soluble vitamins in the TEAC(trolox equivalent antioxidant capacity) and the FRAP (ferric reducing antioxidant power) assays, Food Chemistry 96:131–136.
Güçlü K, Altun M, Özyürek M, Karademir SE, Apak R (2006). Antioxidant capacity of fresh, sun- and sulphited-dried Malatya apricot (Prunus armeniaca) assayed by CUPRAC, ABTS/TEAC and Folin methods, International Journal of Food Science and Technology, 41:76–85.
Hegedüs A, Pfeiffer P, Papp N, László A, Blázovics A, Pedryc A, Stefanovits-Bányai É (2011). Accumulation of Antioxidants in Apricot Fruit through Ripening: Characterization of a Genotype with Enhanced Functional Properties, Biol Res., 44:339-344.
Kallio H, Yang B, Pippo P (2002). Effects of different origins and harvestind time on vitamin C, tocopherols and tocotrienols in sea buckthorn (Hippophae rhamnoides) berries, J Agric Food Chem, 50:6136-6142.
Kruczek M, Świderski A, Mech-Nowak A, Król K (2012). Antioxidant capacity of crude extracts containing carotenoids from the berries of various cultivars of Sea buckthorn (Hippophae rhamnoides L.), 59,1:135-7.
Kurtz C, Carle R, Schieber A (2008). HPLC-DAD-MSncharacterisation of carotenoids from apricots and pumpkins for the evaluation of fruit product authenticity, Food Chem., 2008, 110: 522-530.
Makovics-Zsohár N, Hegedűs A, Stefanovits-Bányai É, Rédei R, Papp N (2014). The antioxidant capacity of sea buckthorn (Hippophae rhamnoides L.) berries depends on the genotype and harvest time, International Journal of Horticultural Science: 27:3-4.
Miller NJ, Sampson J, Candeias LP, Bramley PM et al. (1996). Antioxidant activities of carotenes and xanthophylls.FEBS lett., 384:240–242.
Müller L, Theile K, Böhm V, Schaich K (2010). In vitro antioxidant activity of tocopherols and tocotrienols and comparison of vitamin E concentration and lipophilic antioxidant capacity in human plasma, Mol Nutr Food Res, 54:731-742.
Müller L, Fröhlich K, Böhm V (2011). Comparative antioxidant activities of carotenoids measured by ferric antioxidant power (FRAP), ABTS bleaching assay (αTEAC), DPPH assay and peroxyl radical scavenging assay, Food Chem., 129:139-148.
Obón JM, Castellar MR, Cascales JA, Fernández-López JA (2005). Assessment of the TEAC method for determining the antioxidant capacity of synthetic red food colorants, Food Research International 38:843–845.
Panossian A, Wagner H, Ulrich-Merzenich G (2013). From traditional to evidence-based use of Hippophaë rhamnoides L.: chemical composition, experimental, and clinical pharmacology of sea buckthorn berries and leaves extracts. In Evidence and Rational Based Research on Chinese Drugs, 181–236.
Pintea A, Varga A, Stepnowski P, Socaciu C, Culea M, Horst AD (2005). Chromatographic analysis (HPLC, GC) of Carotenol Fatty Acids Esters in Physalis alkekengi and Hippophae rhamnoides, Phytochemical Analysis, 16:188-195.
Pintea A, Bunea A, Socaciu C (2013). Effect of esterification on thermal stability and antioxidant activity of zeaxanthin, Book of abstracts and proceedings of the 7th International Congress on Pigments in Food, 174-177.
Pintea A, Rugină D, Bunea A, Andrei S (2013b). Impact of Esterification on the Antioxidant Capacity of β-Cryptoxanthin, Bulletin USAMV serie Animal science and Biotechnology, 70(1):79-85.
Prior RL (2003). Fruits and vegetables in the prevention of cellular oxidative damage, Am. J. Clin. Nutr.,78:570-578.
Prior R, Wu X, Schaich K (2005). Standardized Methods for the Determination of Antioxidant Capacity and Phenolics in Foods and Dietary Supplements, J. Agric. Food Chem.,53:4290−4302.
Pop R, Weesepoel Y, Socaciu C, Pintea A, Vincken J-P (2014). Carotenoid composition of berries and leaves from six Romanian Sea Buckthorn (Hippophae rhamnoides L.) varieties, Food Chemistry, Food Chemistry., 147:1-9.
Rao AV, Rao LG (2007). Carotenoids and human health, Pharmacological Research, 55(3):207-216.
Ruiz D, Egea J, Gil MI, Tomas-Barberan FA (2005). Characterization and quantitation of phenolic compounds in new apricot (Prunus armeniaca L.) varieties, J. Agric. Food Chem., 53: 9544-9552.
Ruiz D, Egea J, Tomás-Barberán FA, Gil MI (2005). Carotenoids from new apricot (Prunus armeniaca L.) varieties and their relationship with flesh and skin color, Journal of Agricultural and Food Chemistry, 53:6368−6374.
Ruperez FJ, Martin D, Herrera E, Barbas C (2001). Chromatographic analysis of α-tocopherol and related compounds in various matrices, Journal of Chromatography A, 935: 45-69.
Quesada S, Azofeifa G, Jatunov S, Jiménez G, Navarro L, Gómez G (2011). Carotenoids composition, antioxidant activity and glycemic index of two varieties of Bactris gasipaes, Emir. J. Food Agric. 23: 482-489.
Sass-Kiss A, Kiss J, Milotay P, Kerek MM, Toth-Markus M (2005). Differences in anthocyanin and carotenoid content of fruits and vegetables. Food Research International, 38:1023–1029.
Sharma P, Singh RP (2013) Evaluation of Antioxidant Activity in Foods with Special Reference to TEAC Method. American Journal of Food Technology, 8: 83-101.
Singh S, Singh RP (2008). In Vitro Methods of Assay of Antioxidants: An Overview, Food Reviews International, 24:392-415.
Sochor J, Zitka O, Skutkova H, Pavlik D, Babula P, Krska B, Horna A, Adam V, Provaznik I, Kizek R (2010). Content of Phenolic Compounds and Antioxidant Capacity in Fruits of Apricot Genotypes; Molecules 15: 6285-6305.
Sun J, Chu YF, Wu XZ, Liu RH (2002). Antioxidant and anti proliferative activities of common fruits, J Agric and Food Chem, 50:7449-7454.
Tulipani SG, Marzban A, Herndl M, Mezzetti LB, Battino M (2011). Influence of environmental and genetic factors on health-related compounds in strawberry. Food Chem., 124:906-913.
Turan S, Topcu A, Karabulut I, Vural H, Hayaloglu AA (2007). Fatty acid, triacylglycerol, phytosterol, and tocopherol variations in kernel oil of Malatya apricots from Turkey, J. Agric Food Chem, 55:10787-10794.
Weller P, Breithaupt DE (2003). Identification and quantification of zeaxanthin esters in plants using liquid chromatography-mass spectrometry, J Agric Food Chem., 51:7044-7049.
Yang B, Kallio H (2002). Composition and physiological effects of Sea Buckthorn lipids. Trends Food Sci.Technol., 13:160-167.
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