Molecular identification of six species of Calliphoridae (Diptera) with forensic interest in Bogotá, Colombia

Authors

  • Angela Sabrina Marquez-Acero Universidade de São Paulo (USP) Museu de Zoologia (MZ)
  • Juan Manuel Vidal-Garcia niversidade de São Paulo (USP) Instituto de Matemática e Estadistica (IME)
  • Luis Francisco Becerra Galindo Universidad Distrital Francisco José de Caldas
  • Alexander García García Universidad Distrital Francisco José de Caldas

DOI:

https://doi.org/10.11606/0031-1049.2017.57.12

Keywords:

Forensic entomology, Barcode, mtDNA, COII.

Abstract

Taxonomic identification of the species involved in the processes of cadaveric decomposition is a fundamental procedure in forensic entomological analysis. Among the species involved in the processes of decay, those of the Calliphoridae family are particularly important because they come to the body in the early stages of decomposition. The aim of this research is to identify six species of Calliphoridae (Calliphora nigribasis, Calliphora vicina, Compsomyopsis verena, Sarconesiopsis magellanica, Chrysomia albiceps and Roraimomusca roraima) with forensic interest found in Bogotá. For that, sequences of 599 bp from mitochondrial gene COII wereas obtained. The identification was made by analysis of genetic distances under Jukes-Cantor model. The results showed levels of interspecific distances greater than 3.7%, while intraspecifics levels does not exceed 2.3%. The genetic distances obtained were used to construct a phenogram under the Maximum Likelihood model and the topology of that tree agrees with the current taxonomic organization for the family Calliphoridae family.

Downloads

Download data is not yet available.

References

Alessandrini, F.; Mazzanti, M.; Onofri, V.; Turchi, C. & Tagliabracci, A. 2008. MtDNA analysis for genetic identification of forensically important insects. Forensic Science International: Genetics, Supplement Series, 1:584‑585.

Amat, E.; Velez, M. & Wolff, M. 2008. Clave ilustrada para la identificación de los géneros y las especies de califóridos (Diptera: Calliphoridae) de Colombia. Caldasia, 30(1):231‑244.

Benecke, M. 1998. Six forensic entomology cases: description and commentary. Journal of Forensic Sciences, 43:797‑805; 43:1303.

Boehme, P.; Amendt, J. & Zehner, R. 2012. The use of COI barcodes for molecular identification of forensically important fly species in Germany. Parasitology Research, 110:2325‑2332.

Boehme, P.; Amendt, J.; Disney, H. & Zehner, R. 2010. Molecular identification of carrion-breeding scuttle flies (Diptera: Phoridae) using COI barcodes. International Journal of Legal Medicine, 124(6):577‑581.

Buenaventura, E.; Camacho, G.; García, A. & Wolff, M. 2009. Sarcophagidae (Diptera) de importancia forense en Colombia: claves taxonómicas, notas sobre su biología y distribución. Revista Colombiana de Entomología, 35(2):189‑196.

Cainé, L.; Corte, F.; Saloña-Bordas, M.; Pancorbo, M.; Lima, G.; Magalhaes, T. & Pinheiro, F. 2009. DNA typing of Diptera collected from human corpses in Portugal. Forensic Science International, 184:21‑23.

Camacho, G. 2005. Sucesion de la entomofauna cadaverica y ciclo vital de Calliphora vicina (Diptera: Calliphoridae) como primera especie colonizadora, utilizando cerdo blanco (Sus scrofa) en Bogotá. Revista Colombiana de Entomología, 31(2):189‑197.

Carvalho, C. & Mello-Patiu, C. 2008. Key to the adults of the most common forensic species of Diptera in South America. Revista Brasileira de Entomologia, 52(3):390‑406.

Castalanelli, M.; Severtson, D.; Brumley, C.; Szito, A.; Foottit, R.; Grimm, M.; Munyard, K. & Groth, D. 2010. A rapid non-destructive DNA extraction method for insects and other arthropods. Journal of Asia-Pacific Entomology, 13:243‑248.

Desmyter, S. & Gosselin, M. 2009. COI sequence variability between Chrysomyinae of forensic interest. Forensic Science International: Genetics, 3(2):89‑95.

Elias, M.; Hill, R.; Willmott, K.; Dasmahapatra, K.; Brower, A.; Mallet, J. & Jiggins, C. 2007. Limited performance of DNA barcoding in a diverse community of tropical butterflies. Proceedings of the Royal Society of London B, 274:2881‑2889.

Ferreira, M.J.M. 1978. Sinantropia de dípteros muscóideos em Curitiba, Paraná. I. Calliphoridae. Revista Brasileira de Biologia, 38:445‑454.

Florez, E. & Wolff, M. 2009. Descripción y clave de los estadios inmaduros de las principales especies de Calliphoridae (Diptera) de importancia forense en Colombia. Neotropical Entomology, 38(3):418‑429.

Giraldo, P.; Uribe, S. & López, A. 2011. Análisis de secuencias de ADN mitocondrial (Cytb y ND1) en Lucilia eximia (Diptera: Calliphoridae). Revista Colombiana de Entomología, 37(2):273‑278.

Harvey, M.; Dadour, I. & Gaudieri, S. 2003. Mitochondrial DNA Citochrome oxidase I gene: potential for distinction between immature stages of some forensically important fly species (Diptera) in western Austrália. Forensic Science International, 131:134‑139.

Hebert, P.D.N.; Cywinska, A.; Ball, S.L. & de Waard, J.R. 2003. Biological identifications through DNA barcodes. Proceedings of the Royal Society of London B, 270:313‑321.

Jukes, T.H. & Cantor, C.R. 1969. Evolution of protein molecules. (Munro H N, eel.) Mammalian protein metabolism. III. New York, Academic Press. p. 21‑132.

Junqueira, A.; Lessinger, A.; Torres, T.; Rodrigues da Silva, F.; Vettore, A.; Arruda, P. & Azeredo, A. 2004. The mitochondrial genome of the blowfly Chrysomya chloropyga (Diptera: Calliphoridae). Gene, 339:7‑15.

Meiklejohn, K.; Wallman, J. & Dowton, M. 2011. DNA‑based identification of forensically important Australian Sarcophagidae (Diptera). International Journal of Legal Medicine, 121(1):27‑32.

Nelson, L.; Wallmann, J. & Dowton, M. 2007. Using COI barcodes to identify forensically and medically important blowflies. Medical and Veterinary Entomology, 21:44‑52.

Nelson, L.A.; Lambkin, C.L.; Batterham, P.; Wallman, J.F.; Dowton, M.; Whiting, M.F.; Yeates, D.K. & Cameron, S.L. 2012. Beyond barcoding: A mitochondrial genomics approach to molecular phylogenetics and diagnostics of blowflies (Diptera: Calliphoridae). Gene, 511(2):131‑142.

Oliveira, M.; Barau, J.; Martins, A.; Feijão, P.; Coelho da Rosa, A.; Abreu, C.; Azeredo-Spin, A. & Lessinger, A. 2008. Structure and evolution of the mitochondrial genomes of Haematobia irritans and Stomoxys calcitrans: The Muscidae (Diptera: Calyptratae) perspective. Molecular Phylogenetics and Evolution, 48:850‑857.

Pape, T.; Wolff, M. & Amat, E. 2004. Los califóridos, éstridos, rinofóridos y sarcofágidos (Diptera: Calliphoridae, Oestridae, Rhinophoridae, Sarcophagidae) de Colombia. Biota Colombiana, 5(2):201‑208.

Park, S.; Zhang, Y.; Piao, H.; Ha Yu, D.; Jeong, H.; Young Yoo, G.; Tae‑Ho, J.O. & Hwang, J. 2009. Sequences of the Cytochrome C Oxidase Subunit I (COI) gene are suitable for species identification of Korean Calliphorinae flies of forensic importance (Diptera: Calliphoridae). Journal of Forensic Sciences, 54(5):1131‑1134.

Saigusa, K.; Matsumasa, M.; Yashima, Y.; Takamiya, M. & Aoki, Y. 2009. Practical applications of molecular biological species identification of forensically important flies. Legal Medicine, 11(Supplement 1):344‑347.

Segura, N.; Usaquen, W.; Sanchez, M.; Chuaire, L. & Bello, F. 2009. Succession pattern of cadaverous entomofauna in a semi-rural area of Bogotá, Colombia. Forensic Science international, 187:66‑72.

Tamura, K.; Dudley, J.; Nei, M. & Kumar, S. 2007. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24:1596‑1599.

Thompson, J.; Higgins, D. & Gibson, T. 1994. Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22:4673‑4680.

Tuccia, F.; Giordani, G. & Vanin, S. 2016. A general review of the most commom COI primers for Calliphoridae identification in forensic entomology. Forensic Science International: Genetics, 24:e9‑e11.

Wells, J. & Sperling, F. 2001. DNA‑based identification of forensically important Chrisomyinae (Diptera: Calliphoridae). Forensic Science International, 120:110‑115.

Wells, J. & Stevens, J. 2008. Application of DNA‑Based Methods in Forensic Entomology. Annual Review of Entomology, 53:103‑20.

Wells, J.; Pape, T. & Sperling, F. 2001. DNA‑Based Identification and Molecular Systematics of Forensically Important Sarcophagidae (Diptera). Journal of Forensic Science, 46(5):1098‑1102.

Wells, J.; Wall, R. & Stevens, J.R. 2007. Phylogenetic analysis of forensically important Lucilia flies based on cytochrome oxidase I sequence: a cautionary tale for forensic species determination. International Journal of Legal Medicine, 121:229‑233.

Whitworth, T. 2010. Keys to the genera and species of blow flies (Diptera: Calliphoridae) of the West Indies and description of a new species of Lucilia Robineau-Desvoidy. Zootaxa, 2663:1‑35.

Whitworth, T.; Dawson, R.; Magalon, H. & Baudry, E. 2007. DNA barcoding cannot reliably identify species of the blowfly genus Protocalliphora (Diptera: Calliphoridae). Proceedings of the Royal Society of London B, 274:1731‑1739.

Yusseff, S. 2006. Entomología forense: los insectos en la escena del crimen. Revista Luna Azul, 23:1‑10.

Zehner, R.; Amendt, J. & Shutt, S. 2004. Genetic identification of forensically important flesh flies (Diptera: Sarcophagidae). Journal of Legal Medicine, 118:245‑247.

Downloads

Published

2017-03-16

Issue

Section

Original Article

How to Cite

Marquez-Acero, A. S., Vidal-Garcia, J. M., Galindo, L. F. B., & García, A. G. (2017). Molecular identification of six species of Calliphoridae (Diptera) with forensic interest in Bogotá, Colombia. Papéis Avulsos De Zoologia, 57(12), 141-147. https://doi.org/10.11606/0031-1049.2017.57.12