DNA Fingerprinting Used for Testing Some Romanian Wine Varieties

  • Monica HÂRŢA University of Agriculture Sciences and Veterinary Medicine, Faculty of Horticulture, 3-5 Mănăştur Street, 400372, Cluj-Napoca, Romania;
  • Doru PAMFIL University of Agriculture Sciences and Veterinary Medicine, Faculty of Horticulture, 3-5 Mănăştur Street, 400372, Cluj-Napoca, Romania;
  • Rodica POP University of Agriculture Sciences and Veterinary Medicine, Faculty of Horticulture, 3-5 Mănăştur Street, 400372, Cluj-Napoca, Romania;
  • Simona VICAŞ Faculty of Environmental Protection, University of Oradea, 114-C.P. Universităţii st. Oradea, Romania
DOI: https://doi.org/10.15835/buasvmcn-hort:7041

Abstract

The main goal of the present study was to isolate DNA both experimental and commercial wines in order to differentiate some Romanian grapevine cultivars (Tămâioasă Românescă, Galbenă de Odobeşti, Fetească Neagră and Busuioacă de Bohotin) by PCR using wine as a sample. Extracted residual grape DNA quality and specificity were verified using co-dominant simple sequence repeat (SSR) markers (VVS2, VVMD27, VVMD5 and VVMD7). The results obtained in this research shows that even 18 months after wine fermentation could be detected residual DNA that can be suitable for DNA fingerprinting. The 12-month-old experimental wine samples were more amplified than the 18-month-old ones. Among the microsatellite tested, VVS2 and VVMD7 gave satisfactory results. The VVS2 marker, which generated the shortest PCR amplicons (119-138 bp), amplified all of the cultivars analyzed in the wines samples. In case of commercial wines authentication of grape material by PCR using wine as a biological material was unsuccessfully due to insufficient amount of residual DNA in samples analyzed.
Issue
Vol. 68 No. 1 (2011): Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Horticulture
Section
Research Articles

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