Quercetin potentiates the effect of γδT cells via modulating the expressions of Granzyme B, perforin and IFN-γ and also regulates the Wnt/δ-catenin signalling pathway in human colon cancer cells

Authors

  • Hai-Yan Lu Department of Anorectal, The Second Affiliated Hospital of Hunan Traditional Chinese Medicine University, Changsha, Hunan 410005
  • Tian-Shu Peng Department of Anorectal, The Second Affiliated Hospital of Hunan Traditional Chinese Medicine University, Changsha, Hunan 410005
  • Xiang-Dang Hu Department of Anorectal, The Second Affiliated Hospital of Hunan Traditional Chinese Medicine University, Changsha, Hunan 410005
  • Shuai-Jun Li Department of Anorectal, The Second Affiliated Hospital of Hunan Traditional Chinese Medicine University, Changsha, Hunan 410005
  • Min Luo Department of Anorectal, The Second Affiliated Hospital of Hunan Traditional Chinese Medicine University, Changsha, Hunan 410005
  • Yong-Heng He Department of Anorectal, The Second Affiliated Hospital of Hunan Traditional Chinese Medicine University, Changsha, Hunan 410005
  • Tian Nie Department of Oncology and Blood, The First Affiliated Hospital of Hunan Traditional Chinese Medicine University, Changsha, Hunan 410007

DOI:

https://doi.org/10.3329/bjp.v10i2.20387

Keywords:

Colon cancer, Quercetin, Wnt/?-catenin signaling pathway, ?? T cells

Abstract

Cancer accounts as one of the leading causes of morbidity and mortality. Recent studies focus on the efficiency of phytochemicals in cancer therapy. Influence of quercetin, a flavonoid on the effect of γδ T cells and Wnt/β-catenin signalling pathway in human colon cancer cells (HT55 and HCT116) was investigated. Quercetin at 15-120 µM was observed to markedly reduce the viability of HT55 and HCT116 cells. Quercetin exposure significantly increased γδ T cell proliferation and also raised the expressions of granzyme B (Gra B), perforin (PFP), and interferon- γ (IFN-γ) in γδ T cells. Reduced β-catenin expression with increased expressions of phosphorylated- β-catenin, axin1 and 2 were observed in HT55 and HCT116 cells on exposure to quercetin. However β-actin expression was found to be not much altered. The results suggest that quercetin was able to efficiently potentiate the effect of γδ T cells and modulate Wnt/β-catenin signalling pathway. 

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Published

2015-04-01

How to Cite

Lu, H.-Y., T.-S. Peng, X.-D. Hu, S.-J. Li, M. Luo, Y.-H. He, and T. Nie. “Quercetin Potentiates the Effect of γδT Cells via Modulating the Expressions of Granzyme B, Perforin and IFN-γ and Also Regulates the Wnt/δ-Catenin Signalling Pathway in Human Colon Cancer Cells”. Bangladesh Journal of Pharmacology, vol. 10, no. 2, Apr. 2015, pp. 251-9, doi:10.3329/bjp.v10i2.20387.

Issue

Vol. 10 No. 2 (2015)

Section

Research Articles

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