The effect of the new combined cream on the protein synthetic activity of cells on the model of the thermal skin damage in rats

Authors

  • N. M. Kononenko National University of Pharmacy, Ukraine
  • A. M. Sheikhali National University of Pharmacy, Ukraine
  • M. O. Ostapets National University of Pharmacy, Ukraine
  • V. S. Loboda National University of Pharmacy, Ukraine

DOI:

https://doi.org/10.24959/nphj.17.2192

Keywords:

burns, cream, α-lipoic acid, reparation, ribonucleoproteins, dezoxyribonucleoproteins

Abstract

Aim. To study the mechanisms of the reparative action of a new combined cream on the model of thermal burn injury in rats.

Materials and methods. On the model of thermal burn in rats the reparative properties of the new combined cream under the conditional name “Dermalipoin” containing α-lipoic acid, urea, olive oil, tea tree oil, PEG-400 were studied. To determine the level of the protein synthetic activity of cells (epitheliocytes of the stratified squamous epithelium in the epidermis and cells of the fibroblast cell in the edges of healing of thermal damage) the content of ribonucleoproteins (RNP) in the cytoplasm and deoxyribonucleoproteins (DNP) in the nuclei of the cells was assessed by the cytometric method.

Results and discussion. On the model of thermal burn in rats “Dermalipoin” cream showed a more active effect on reparative processes compared to the reference drugs – methyluracil ointment and “Titriol” gel. It indicates a significant increase in the protein synthetic function of epitheliocytes of the stratified squamous epithelium in the epidermis and cells of the fibroblastic series at the healing edges. The reparative activity of the cream was, first of all, provided by the presence of thiol (sulfhydryl) groups in the molecule of lipoic acid, giving it the properties of an antioxidant. The antioxidant effect of lipoic acid promotes more efficient DNA molecule reparation after damage as a result of the oxidative stress.

Conclusions. “Dermalipoin” cream shows a marked reparative effect due to the increase of the protein synthetic activity and acceleration of reparation of DNA molecules of epithelial cells after damage; as a result, the process of healing burns accelerates.

Author Biographies

N. M. Kononenko, National University of Pharmacy

Doctor of Medicine (Dr. habil.), professor, head of the Department of Pathological Physiology

A. M. Sheikhali, National University of Pharmacy

postgraduate student of the Department of Pathological Physiology

M. O. Ostapets, National University of Pharmacy

Candidate of Medicine (PhD), teaching assistant of the Department of Pathological Physiology

V. S. Loboda, National University of Pharmacy

Candidate of Physical Education and Sports (PhD), head of the Department of Physical Education and Health

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Published

2017-12-04

Issue

No. 4(92) (2017)

Section

Experimental and Clinical Pharmacology

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Copyright (c) 2017 National University of Pharmacy

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