Production Improvement of M1 Generation Garut (Maranta arundinacea) Rhizomes through Gamma Radiation Mutation

Puspita Deswina(1), Dody Priadi(2), Yashanti Berlinda Paradisa(3), Yuliana Galih Dyan Anggraheni(4), Enung Sri Mulyaningsih(5), Ambar Yuswi Perdani(6), Sri Indrayani(7),


(1) Research Center for Biotechnology - LIPI
(2) Research Center for Biotechnology - LIPI
(3) Research Center for Biotechnology - LIPI
(4) Research Center for Biotechnology - LIPI
(5) Research Center for Biotechnology - LIPI
(6) Research Center for Biotechnology - LIPI
(7) Research Center for Biotechnology - LIPI

Abstract

Garut (Maranta arundinacea L.) is a tuber containing flour with a low Glycemic Index (IG) suitable for diabetics and other degenerative diseases. It needs to be developed to reduce the dependence on imports or to substitute wheat flour. The objective of the study was to obtain superior arrowroot seedlings and observe the effect of gamma-ray irradiation on the density and position of stomata on arrowroot leaves. In this research, the irradiation of five arrowroot accessions of second-generation (MV1) with gamma rays (0, 10, 20, 30, 40, and 50 Gray) was carried out to obtain mutants with superior character so that they could be used as parent plants for arrowroot flour production. The treatments administered were arrowroot accessions (V), gamma-ray radiation dose (R), and their interaction. The qualitative and quantitative characters on plant characteristics, tuber production, and arrowroot leaves' stomata were observed. The research results showed that increased production is primarily for 25-Pandeglang accession (808.33 grams) and 10 Gray (800.00 grams) of gamma ray radiation treatment. It is expected that this accession can be released as a new variety candidate after subsequent selection and evaluation in a further generation. Moreover, the dose of gamma-ray radiation is inversely proportional to the number of stomata, which will increase the photosynthesis, thus increasing the number of tubers produced.

Keywords

arrowroot, diversity, gamma-ray radiation, garut rhizome

Full Text:

PDF

References

Aisyah, S. I., & Darusman, L. K. (2014). Induksi mutasi fisik dengan iradiasi sinar gamma pada kunyit (Curcuma domestica Val.). Jurnal Hortikultura Indonesia, 5(2), 84-94.

Asha, K. I., Krishna Radhika, N., Vineetha, B., Asha Devi, A., Sheela, M. N., & Sreekumar, J. (2015). Diversity analysis of arrowroot germplasm using ISSR markers.Journal of Root Crops, 41 (1).17-24.

Cahyana, Y., Rangkuti, A., Siti Halimah, T., Marta, H., & Yuliana, T. (2020). Application of heat-moisture-treated banana flour as composite material in hard biscuit. CyTA-Journal of Food, 18(1), 599-605.

Elvandari, N., Winarti, S. & Sarofa, U. (2020, March). Glycemic index of biscuit non-wheat from mangrove fruits flour with arrowroot and canna flours. In the 5th International Conference on Food, Agriculture and Natural Resources (FANRes 2019) (pp. 213-218). Atlantis Press.

Garvita, R. V., & Wawangningrum, H. (2020). Stomata cells studies of Paraphalaenopsis spp. from in vitro and greenhouse conditions. Biodiversitas Journal of Biological Diversity, 21(3), 1116-1121.

Hasan, V., Astuti, S. & Susilawati, S. (2012). Indeks glikemik oyek dan tiwul dari umbi garut (Maranta arundinacea L.), suweg (Amorphophallus campanulatus BI) dan singkong (Manihot utilissima). Jurnal Teknologi & Industri Hasil Pertanian, 16(1), 34-50.

He, Y., Zhou, K., Wu, Z., Li, B., Fu, J., Lin, C., & Jiang, D. (2019). Highly efficient nanoscale analysis of plant stomata and cell surface using polyaddition silicone rubber. Frontiers in plant science, 10, 1569.

Hidayati, N., Sukamto, L. A., & Juhaeti, T. (2012). Pengujian ketahanan kekeringan pada tanaman garut (Maranta arundinacea L.) hasil mutasi dengan radiasi sinar gamma. Jurnal Biologi Indonesia, 8(2), 303-315.

Kaur, R., Kapoor, M., Kaur, R., & Kumar, A. (2017). Effect of gamma irradiation on cyto-morphology, total phenolic content, and antioxidant activity of calendula. Journal of Hill Agriculture, 8(4), 395-402.

Klarisya, L., & Daningsih, E. (2021). Stomatal number and size of ornamental dicotyledons plant in Pontianak west kalimantan. Proceedings of KOBI 2nd International Confer, 1, 61-66.

Lim, T. K. (2012). Edible medicinal and non-medicinal plants (Vol. 1, pp. 285-292). Dordrecht, The Netherlands: Springer.

Marteen, A., Hussain, S., Rehman, S. U., Mahmood, B., Khan, M. A., Rashid, A., Sohail, M., Farooq, M. & Shah, S. J. A. (2012). Suitability of various plant-derived gelling agents as agar substitutes in microbiological growth media. African Journal of Biotechnology, 11(45), 10362-10367.

Oktavianingsih L., Suharyanto, E., Daryono, B. S., & Purnomo. (2017). Traditional usages of taro (Colocasia spp.) by ethnic communities in Borneo. Biosaintifika: Journal of Biology & Biology Education, 9(2), 248-256

Rohandi, A., Budiadi, B., Hardiwinoto, S., Harmayani, E., & Sudrajat, D. J. (2017). Variability in morpho-physiology, tuber yield and starch content of several arrowroot populations in Garut district. Agrivita, Journal of Agricultural Science, 39(3), 311-323.

Rosmaina, Zulhirwan, R., Suryani, P., Irfan, M. & Zulfahmi. (2019). Penurunan kandungan klorofil dan kerusakan stomata akibat cekaman suhu tinggi tanaman cabai merah (Capsicum annuum L.) pada fase juvenil. Prosiding Semirata BKS-PTN Wilayah Barat Bidang Ilmu Pertanian “Inovasi Pertanian Berbasis Sumberdaya Lokal Berorientasi Entrepreneurship”

Saragih, S.H.Y.,Rizal, K., Sitanggang, K.D. (2020). Induksi mutasi kara benguk (Mucuna pruriens L.) menggunakan iradiasi sinar gamma. Jurnal Penelitian Agronomi (Agrosains). 22(2), 105-108.

Sholichah, E., Deswina, P., Sarifudin, A., Andriansyah, C. E. & Rahman, N. (2019). Physicochemical, structural and morphological properties of some arrowroot (Maranta arundinacea) accessions growth in Indonesia. In AIP Conference Proceedings (Vol. 2175, No. 1, p. 020008). AIP Publishing LLC.

Sukamto, L. A., Wawo, A. H. & Ahmad, F. (2016). Pengaruh oryzalin terhadap tingkat ploidi tanaman garut (Maranta arundinacea L.). Buletin Penelitian Tanaman Rempah dan Obat, 21(2), 93-102.

Sreelakshmi, V. V., Sruthy, E. P. M. & Shereena, J. (2014). Relationship between the leaf area and taxonomic importance of foliar stomata. International Journal of Research in Applied, Natural and Social Sciences, 2(7), 53-60.

Suhartini, T. (2016). Keragaman karakter morfologis garut (Maranta arundinacea L.). Buletin Plasma Nutfah, 17(1), 12-18.

Suryanti, S. & Umami, A. (2020). Stomata dan trikoma kultivar kedelai anjasmoro selama pemupukan nanosilika dan plant growth promoting rhizobacteria. Vegetalika, 9(1), 343-349.

Refbacks

  • There are currently no refbacks.




Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.